mouse anti-cc1 op80 Search Results


mouse anti-cc1 (ihc 1:200 in the analysis of development, 1:300 in the analysis of remyelination, cat# op80  (Merck KGaA)
90
Merck KGaA mouse anti-cc1 (ihc 1:200 in the analysis of development, 1:300 in the analysis of remyelination, cat# op80
Mouse Anti Cc1 (Ihc 1:200 In The Analysis Of Development, 1:300 In The Analysis Of Remyelination, Cat# Op80, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti-cc1+op80/anti+apc++1+300++op80+/10__7554_slash_elife__44702-288-20-37
Average 90 stars, based on 1 article reviews
mouse anti-cc1 (ihc 1:200 in the analysis of development, 1:300 in the analysis of remyelination, cat# op80 - by Bioz Stars, 2026-10
90/100 stars
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mouse anti-apc  (Merck KGaA)
90
Merck KGaA mouse anti-apc
Mouse Anti Apc, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti-cc1+op80/mouse+anti+apc/pm24647135-374-29-33
Average 90 stars, based on 1 article reviews
mouse anti-apc - by Bioz Stars, 2026-10
90/100 stars
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anti apc  (Santa Cruz Biotechnology)
94
Santa Cruz Biotechnology anti apc
Anti Apc, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti-cc1+op80/APC+Antibody/10__1523_slash_jneurosci__0211___16__2016-121-18-14
Average 94 stars, based on 1 article reviews
anti apc - by Bioz Stars, 2026-10
94/100 stars
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mouse anti-cc1  (Merck KGaA)
90
Merck KGaA mouse anti-cc1

Mouse Anti Cc1, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti-cc1+op80/antibody+mouse+monoclonal+anti+cc1/pmc06504237-262-22-39
Average 90 stars, based on 1 article reviews
mouse anti-cc1 - by Bioz Stars, 2026-10
90/100 stars
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anti adenomatous polyposis coli  (R&D Systems)
96
R&D Systems anti adenomatous polyposis coli
Neuronal activity-dependent Ca 2+ activities in oligodendrocytes (OCs). (A,B) Representative images (A) and quantification (B) of GCaMP expression in the motor cortex of PLP-GCaMP mice and co-localization with markers for OC + oligodendrocyte precursor cell (OPC) (Olig2), OC <t>(CC1),</t> OPC (PDGFRα, NG2), neuron (NeuN), astrocyte (S100β) (Olig2 [83.41 ± 3.899%], CC1 [72.65 ± 3.464%], PDGFRα+ [9.979 ± 0.9322%], NG2 [6.736 ± 0.2401%], NeuN [1.481 ± 0.7246%], and S100β [8.156 ± 0.5504%]). Scale bar, 30 μm. (C,D) Representative images of MBP immunostaining in the motor cortex of PLP-GCaMP6 mice. Scale bars: in (C) , 100 μm; in (D) , 30 μm. (E) Quantitative analysis of the co-localization areas of MBP and GCaMP’ + ve processes based on immunostaining data. The proportion of MBP’ + ve processes in the processes of GCaMP’ + ve cells was about 15%. (F) Experimental protocol of Ca 2+ imaging in OCs. The first craniotomy was performed one week before Pre-imaging (Pre) using two-photon microscopy. After Pre-imaging, a second craniotomy was performed and TTX was applied for 30 min, followed by a second imaging (TTX) of the same cells. (G,H) Representative image of GCaMP’ + ve cells of the motor cortex in PLP-GCaMP6 mice before and after TTX application. Red spots indicate the Ca 2+ activated areas (spot). Scale bar = 30 μm. Representative Ca 2+ traces from spots of typical GCaMP’ + ve cells are shown. (I) Changes in Ca 2+ spots, Ca 2+ events and total area under the curve (AUC) of GCaMP’ + ve cells between before and after TTX application. The number of Ca 2+ spots and Ca 2+ events and total AUC were significantly decreased after TTX application. Pre: n = 6 mice, 13 imaging fields (cells); TTX: n = 6 mice, 13 imaging fields (cells). * p < 0.05, ** p < 0.01, Mann–Whitney U test. Data are presented as mean ± standard error of mean. For detailed data, check the source data file. (J) Changes in AUC, Amplitude, and Latency of GCaMP’ + ve cells between before and after TTX application. AUC was not significantly changed, Amplitude was significantly decreased, and Latency was significantly increased after TTX application. Pre: n = 6 mice, 426 events; TTX: n = 6 mice, 189 events. N.S., not significant, * p < 0.05, *** p < 0.001, Mann–Whitney U test. Violin plots show median (black line) and distribution of the data. For detailed data, check the source data file. (K) Proportions of AUC and Latency were not significantly changed between before and after TTX application. The proportion of lower Amplitude was significantly increased after TTX application. N.S., not significant, ** p < 0.01, Kolmogorov–Smirnov test. For detailed data, check the source data file.
Anti Adenomatous Polyposis Coli, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti-cc1+op80/Mouse+PDGF+R+alpha+Antibody/pmc10644703-90-15-26
Average 96 stars, based on 1 article reviews
anti adenomatous polyposis coli - by Bioz Stars, 2026-10
96/100 stars
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mouse monoclonal anti apc  (Merck & Co)
86
Merck & Co mouse monoclonal anti apc
Neuronal activity-dependent Ca 2+ activities in oligodendrocytes (OCs). (A,B) Representative images (A) and quantification (B) of GCaMP expression in the motor cortex of PLP-GCaMP mice and co-localization with markers for OC + oligodendrocyte precursor cell (OPC) (Olig2), OC <t>(CC1),</t> OPC (PDGFRα, NG2), neuron (NeuN), astrocyte (S100β) (Olig2 [83.41 ± 3.899%], CC1 [72.65 ± 3.464%], PDGFRα+ [9.979 ± 0.9322%], NG2 [6.736 ± 0.2401%], NeuN [1.481 ± 0.7246%], and S100β [8.156 ± 0.5504%]). Scale bar, 30 μm. (C,D) Representative images of MBP immunostaining in the motor cortex of PLP-GCaMP6 mice. Scale bars: in (C) , 100 μm; in (D) , 30 μm. (E) Quantitative analysis of the co-localization areas of MBP and GCaMP’ + ve processes based on immunostaining data. The proportion of MBP’ + ve processes in the processes of GCaMP’ + ve cells was about 15%. (F) Experimental protocol of Ca 2+ imaging in OCs. The first craniotomy was performed one week before Pre-imaging (Pre) using two-photon microscopy. After Pre-imaging, a second craniotomy was performed and TTX was applied for 30 min, followed by a second imaging (TTX) of the same cells. (G,H) Representative image of GCaMP’ + ve cells of the motor cortex in PLP-GCaMP6 mice before and after TTX application. Red spots indicate the Ca 2+ activated areas (spot). Scale bar = 30 μm. Representative Ca 2+ traces from spots of typical GCaMP’ + ve cells are shown. (I) Changes in Ca 2+ spots, Ca 2+ events and total area under the curve (AUC) of GCaMP’ + ve cells between before and after TTX application. The number of Ca 2+ spots and Ca 2+ events and total AUC were significantly decreased after TTX application. Pre: n = 6 mice, 13 imaging fields (cells); TTX: n = 6 mice, 13 imaging fields (cells). * p < 0.05, ** p < 0.01, Mann–Whitney U test. Data are presented as mean ± standard error of mean. For detailed data, check the source data file. (J) Changes in AUC, Amplitude, and Latency of GCaMP’ + ve cells between before and after TTX application. AUC was not significantly changed, Amplitude was significantly decreased, and Latency was significantly increased after TTX application. Pre: n = 6 mice, 426 events; TTX: n = 6 mice, 189 events. N.S., not significant, * p < 0.05, *** p < 0.001, Mann–Whitney U test. Violin plots show median (black line) and distribution of the data. For detailed data, check the source data file. (K) Proportions of AUC and Latency were not significantly changed between before and after TTX application. The proportion of lower Amplitude was significantly increased after TTX application. N.S., not significant, ** p < 0.01, Kolmogorov–Smirnov test. For detailed data, check the source data file.
Mouse Monoclonal Anti Apc, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti-cc1+op80/anti+flag+%CE%B1/bio_rxiv__2025__10__27__684952-47-1-8
Average 86 stars, based on 1 article reviews
mouse monoclonal anti apc - by Bioz Stars, 2026-10
86/100 stars
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mouse anti cc1  (Merck & Co)
86
Merck & Co mouse anti cc1
Neuronal activity-dependent Ca 2+ activities in oligodendrocytes (OCs). (A,B) Representative images (A) and quantification (B) of GCaMP expression in the motor cortex of PLP-GCaMP mice and co-localization with markers for OC + oligodendrocyte precursor cell (OPC) (Olig2), OC <t>(CC1),</t> OPC (PDGFRα, NG2), neuron (NeuN), astrocyte (S100β) (Olig2 [83.41 ± 3.899%], CC1 [72.65 ± 3.464%], PDGFRα+ [9.979 ± 0.9322%], NG2 [6.736 ± 0.2401%], NeuN [1.481 ± 0.7246%], and S100β [8.156 ± 0.5504%]). Scale bar, 30 μm. (C,D) Representative images of MBP immunostaining in the motor cortex of PLP-GCaMP6 mice. Scale bars: in (C) , 100 μm; in (D) , 30 μm. (E) Quantitative analysis of the co-localization areas of MBP and GCaMP’ + ve processes based on immunostaining data. The proportion of MBP’ + ve processes in the processes of GCaMP’ + ve cells was about 15%. (F) Experimental protocol of Ca 2+ imaging in OCs. The first craniotomy was performed one week before Pre-imaging (Pre) using two-photon microscopy. After Pre-imaging, a second craniotomy was performed and TTX was applied for 30 min, followed by a second imaging (TTX) of the same cells. (G,H) Representative image of GCaMP’ + ve cells of the motor cortex in PLP-GCaMP6 mice before and after TTX application. Red spots indicate the Ca 2+ activated areas (spot). Scale bar = 30 μm. Representative Ca 2+ traces from spots of typical GCaMP’ + ve cells are shown. (I) Changes in Ca 2+ spots, Ca 2+ events and total area under the curve (AUC) of GCaMP’ + ve cells between before and after TTX application. The number of Ca 2+ spots and Ca 2+ events and total AUC were significantly decreased after TTX application. Pre: n = 6 mice, 13 imaging fields (cells); TTX: n = 6 mice, 13 imaging fields (cells). * p < 0.05, ** p < 0.01, Mann–Whitney U test. Data are presented as mean ± standard error of mean. For detailed data, check the source data file. (J) Changes in AUC, Amplitude, and Latency of GCaMP’ + ve cells between before and after TTX application. AUC was not significantly changed, Amplitude was significantly decreased, and Latency was significantly increased after TTX application. Pre: n = 6 mice, 426 events; TTX: n = 6 mice, 189 events. N.S., not significant, * p < 0.05, *** p < 0.001, Mann–Whitney U test. Violin plots show median (black line) and distribution of the data. For detailed data, check the source data file. (K) Proportions of AUC and Latency were not significantly changed between before and after TTX application. The proportion of lower Amplitude was significantly increased after TTX application. N.S., not significant, ** p < 0.01, Kolmogorov–Smirnov test. For detailed data, check the source data file.
Mouse Anti Cc1, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti-cc1+op80/anti+cc1/pm38751168-123-30-37
Average 86 stars, based on 1 article reviews
mouse anti cc1 - by Bioz Stars, 2026-10
86/100 stars
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human sox10 antibody  (Bio-Techne corporation)
96
Bio-Techne corporation human sox10 antibody
Neuronal activity-dependent Ca 2+ activities in oligodendrocytes (OCs). (A,B) Representative images (A) and quantification (B) of GCaMP expression in the motor cortex of PLP-GCaMP mice and co-localization with markers for OC + oligodendrocyte precursor cell (OPC) (Olig2), OC <t>(CC1),</t> OPC (PDGFRα, NG2), neuron (NeuN), astrocyte (S100β) (Olig2 [83.41 ± 3.899%], CC1 [72.65 ± 3.464%], PDGFRα+ [9.979 ± 0.9322%], NG2 [6.736 ± 0.2401%], NeuN [1.481 ± 0.7246%], and S100β [8.156 ± 0.5504%]). Scale bar, 30 μm. (C,D) Representative images of MBP immunostaining in the motor cortex of PLP-GCaMP6 mice. Scale bars: in (C) , 100 μm; in (D) , 30 μm. (E) Quantitative analysis of the co-localization areas of MBP and GCaMP’ + ve processes based on immunostaining data. The proportion of MBP’ + ve processes in the processes of GCaMP’ + ve cells was about 15%. (F) Experimental protocol of Ca 2+ imaging in OCs. The first craniotomy was performed one week before Pre-imaging (Pre) using two-photon microscopy. After Pre-imaging, a second craniotomy was performed and TTX was applied for 30 min, followed by a second imaging (TTX) of the same cells. (G,H) Representative image of GCaMP’ + ve cells of the motor cortex in PLP-GCaMP6 mice before and after TTX application. Red spots indicate the Ca 2+ activated areas (spot). Scale bar = 30 μm. Representative Ca 2+ traces from spots of typical GCaMP’ + ve cells are shown. (I) Changes in Ca 2+ spots, Ca 2+ events and total area under the curve (AUC) of GCaMP’ + ve cells between before and after TTX application. The number of Ca 2+ spots and Ca 2+ events and total AUC were significantly decreased after TTX application. Pre: n = 6 mice, 13 imaging fields (cells); TTX: n = 6 mice, 13 imaging fields (cells). * p < 0.05, ** p < 0.01, Mann–Whitney U test. Data are presented as mean ± standard error of mean. For detailed data, check the source data file. (J) Changes in AUC, Amplitude, and Latency of GCaMP’ + ve cells between before and after TTX application. AUC was not significantly changed, Amplitude was significantly decreased, and Latency was significantly increased after TTX application. Pre: n = 6 mice, 426 events; TTX: n = 6 mice, 189 events. N.S., not significant, * p < 0.05, *** p < 0.001, Mann–Whitney U test. Violin plots show median (black line) and distribution of the data. For detailed data, check the source data file. (K) Proportions of AUC and Latency were not significantly changed between before and after TTX application. The proportion of lower Amplitude was significantly increased after TTX application. N.S., not significant, ** p < 0.01, Kolmogorov–Smirnov test. For detailed data, check the source data file.
Human Sox10 Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti-cc1+op80/Human+SOX10+Antibody/custom%40af2864%4039763780
Average 96 stars, based on 1 article reviews
human sox10 antibody - by Bioz Stars, 2026-10
96/100 stars
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mouse pdgf r alpha antibody  (Bio-Techne corporation)
98
Bio-Techne corporation mouse pdgf r alpha antibody
Neuronal activity-dependent Ca 2+ activities in oligodendrocytes (OCs). (A,B) Representative images (A) and quantification (B) of GCaMP expression in the motor cortex of PLP-GCaMP mice and co-localization with markers for OC + oligodendrocyte precursor cell (OPC) (Olig2), OC <t>(CC1),</t> OPC (PDGFRα, NG2), neuron (NeuN), astrocyte (S100β) (Olig2 [83.41 ± 3.899%], CC1 [72.65 ± 3.464%], PDGFRα+ [9.979 ± 0.9322%], NG2 [6.736 ± 0.2401%], NeuN [1.481 ± 0.7246%], and S100β [8.156 ± 0.5504%]). Scale bar, 30 μm. (C,D) Representative images of MBP immunostaining in the motor cortex of PLP-GCaMP6 mice. Scale bars: in (C) , 100 μm; in (D) , 30 μm. (E) Quantitative analysis of the co-localization areas of MBP and GCaMP’ + ve processes based on immunostaining data. The proportion of MBP’ + ve processes in the processes of GCaMP’ + ve cells was about 15%. (F) Experimental protocol of Ca 2+ imaging in OCs. The first craniotomy was performed one week before Pre-imaging (Pre) using two-photon microscopy. After Pre-imaging, a second craniotomy was performed and TTX was applied for 30 min, followed by a second imaging (TTX) of the same cells. (G,H) Representative image of GCaMP’ + ve cells of the motor cortex in PLP-GCaMP6 mice before and after TTX application. Red spots indicate the Ca 2+ activated areas (spot). Scale bar = 30 μm. Representative Ca 2+ traces from spots of typical GCaMP’ + ve cells are shown. (I) Changes in Ca 2+ spots, Ca 2+ events and total area under the curve (AUC) of GCaMP’ + ve cells between before and after TTX application. The number of Ca 2+ spots and Ca 2+ events and total AUC were significantly decreased after TTX application. Pre: n = 6 mice, 13 imaging fields (cells); TTX: n = 6 mice, 13 imaging fields (cells). * p < 0.05, ** p < 0.01, Mann–Whitney U test. Data are presented as mean ± standard error of mean. For detailed data, check the source data file. (J) Changes in AUC, Amplitude, and Latency of GCaMP’ + ve cells between before and after TTX application. AUC was not significantly changed, Amplitude was significantly decreased, and Latency was significantly increased after TTX application. Pre: n = 6 mice, 426 events; TTX: n = 6 mice, 189 events. N.S., not significant, * p < 0.05, *** p < 0.001, Mann–Whitney U test. Violin plots show median (black line) and distribution of the data. For detailed data, check the source data file. (K) Proportions of AUC and Latency were not significantly changed between before and after TTX application. The proportion of lower Amplitude was significantly increased after TTX application. N.S., not significant, ** p < 0.01, Kolmogorov–Smirnov test. For detailed data, check the source data file.
Mouse Pdgf R Alpha Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti-cc1+op80/Mouse+PDGF+R+alpha+Antibody/custom%40af1062%4031753579
Average 98 stars, based on 1 article reviews
mouse pdgf r alpha antibody - by Bioz Stars, 2026-10
98/100 stars
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human/mouse carbonic anhydrase ii/ca2 antibody  (Bio-Techne corporation)
93
Bio-Techne corporation human/mouse carbonic anhydrase ii/ca2 antibody
Neuronal activity-dependent Ca 2+ activities in oligodendrocytes (OCs). (A,B) Representative images (A) and quantification (B) of GCaMP expression in the motor cortex of PLP-GCaMP mice and co-localization with markers for OC + oligodendrocyte precursor cell (OPC) (Olig2), OC <t>(CC1),</t> OPC (PDGFRα, NG2), neuron (NeuN), astrocyte (S100β) (Olig2 [83.41 ± 3.899%], CC1 [72.65 ± 3.464%], PDGFRα+ [9.979 ± 0.9322%], NG2 [6.736 ± 0.2401%], NeuN [1.481 ± 0.7246%], and S100β [8.156 ± 0.5504%]). Scale bar, 30 μm. (C,D) Representative images of MBP immunostaining in the motor cortex of PLP-GCaMP6 mice. Scale bars: in (C) , 100 μm; in (D) , 30 μm. (E) Quantitative analysis of the co-localization areas of MBP and GCaMP’ + ve processes based on immunostaining data. The proportion of MBP’ + ve processes in the processes of GCaMP’ + ve cells was about 15%. (F) Experimental protocol of Ca 2+ imaging in OCs. The first craniotomy was performed one week before Pre-imaging (Pre) using two-photon microscopy. After Pre-imaging, a second craniotomy was performed and TTX was applied for 30 min, followed by a second imaging (TTX) of the same cells. (G,H) Representative image of GCaMP’ + ve cells of the motor cortex in PLP-GCaMP6 mice before and after TTX application. Red spots indicate the Ca 2+ activated areas (spot). Scale bar = 30 μm. Representative Ca 2+ traces from spots of typical GCaMP’ + ve cells are shown. (I) Changes in Ca 2+ spots, Ca 2+ events and total area under the curve (AUC) of GCaMP’ + ve cells between before and after TTX application. The number of Ca 2+ spots and Ca 2+ events and total AUC were significantly decreased after TTX application. Pre: n = 6 mice, 13 imaging fields (cells); TTX: n = 6 mice, 13 imaging fields (cells). * p < 0.05, ** p < 0.01, Mann–Whitney U test. Data are presented as mean ± standard error of mean. For detailed data, check the source data file. (J) Changes in AUC, Amplitude, and Latency of GCaMP’ + ve cells between before and after TTX application. AUC was not significantly changed, Amplitude was significantly decreased, and Latency was significantly increased after TTX application. Pre: n = 6 mice, 426 events; TTX: n = 6 mice, 189 events. N.S., not significant, * p < 0.05, *** p < 0.001, Mann–Whitney U test. Violin plots show median (black line) and distribution of the data. For detailed data, check the source data file. (K) Proportions of AUC and Latency were not significantly changed between before and after TTX application. The proportion of lower Amplitude was significantly increased after TTX application. N.S., not significant, ** p < 0.01, Kolmogorov–Smirnov test. For detailed data, check the source data file.
Human/Mouse Carbonic Anhydrase Ii/Ca2 Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti-cc1+op80/Human%2FMouse+Carbonic+Anhydrase+II%2FCA2+Antibody/bio-techne+corporation___mab2184
Average 93 stars, based on 1 article reviews
human/mouse carbonic anhydrase ii/ca2 antibody - by Bioz Stars, 2026-10
93/100 stars
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gfap antibody (ga-5)  (Bio-Techne corporation)
94
Bio-Techne corporation gfap antibody (ga-5)
Neuronal activity-dependent Ca 2+ activities in oligodendrocytes (OCs). (A,B) Representative images (A) and quantification (B) of GCaMP expression in the motor cortex of PLP-GCaMP mice and co-localization with markers for OC + oligodendrocyte precursor cell (OPC) (Olig2), OC <t>(CC1),</t> OPC (PDGFRα, NG2), neuron (NeuN), astrocyte (S100β) (Olig2 [83.41 ± 3.899%], CC1 [72.65 ± 3.464%], PDGFRα+ [9.979 ± 0.9322%], NG2 [6.736 ± 0.2401%], NeuN [1.481 ± 0.7246%], and S100β [8.156 ± 0.5504%]). Scale bar, 30 μm. (C,D) Representative images of MBP immunostaining in the motor cortex of PLP-GCaMP6 mice. Scale bars: in (C) , 100 μm; in (D) , 30 μm. (E) Quantitative analysis of the co-localization areas of MBP and GCaMP’ + ve processes based on immunostaining data. The proportion of MBP’ + ve processes in the processes of GCaMP’ + ve cells was about 15%. (F) Experimental protocol of Ca 2+ imaging in OCs. The first craniotomy was performed one week before Pre-imaging (Pre) using two-photon microscopy. After Pre-imaging, a second craniotomy was performed and TTX was applied for 30 min, followed by a second imaging (TTX) of the same cells. (G,H) Representative image of GCaMP’ + ve cells of the motor cortex in PLP-GCaMP6 mice before and after TTX application. Red spots indicate the Ca 2+ activated areas (spot). Scale bar = 30 μm. Representative Ca 2+ traces from spots of typical GCaMP’ + ve cells are shown. (I) Changes in Ca 2+ spots, Ca 2+ events and total area under the curve (AUC) of GCaMP’ + ve cells between before and after TTX application. The number of Ca 2+ spots and Ca 2+ events and total AUC were significantly decreased after TTX application. Pre: n = 6 mice, 13 imaging fields (cells); TTX: n = 6 mice, 13 imaging fields (cells). * p < 0.05, ** p < 0.01, Mann–Whitney U test. Data are presented as mean ± standard error of mean. For detailed data, check the source data file. (J) Changes in AUC, Amplitude, and Latency of GCaMP’ + ve cells between before and after TTX application. AUC was not significantly changed, Amplitude was significantly decreased, and Latency was significantly increased after TTX application. Pre: n = 6 mice, 426 events; TTX: n = 6 mice, 189 events. N.S., not significant, * p < 0.05, *** p < 0.001, Mann–Whitney U test. Violin plots show median (black line) and distribution of the data. For detailed data, check the source data file. (K) Proportions of AUC and Latency were not significantly changed between before and after TTX application. The proportion of lower Amplitude was significantly increased after TTX application. N.S., not significant, ** p < 0.01, Kolmogorov–Smirnov test. For detailed data, check the source data file.
Gfap Antibody (Ga 5), supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
gfap antibody (ga-5) - by Bioz Stars, 2026-10
94/100 stars
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aif-1/iba1 antibody  (Bio-Techne corporation)
99
Bio-Techne corporation aif-1/iba1 antibody
Neuronal activity-dependent Ca 2+ activities in oligodendrocytes (OCs). (A,B) Representative images (A) and quantification (B) of GCaMP expression in the motor cortex of PLP-GCaMP mice and co-localization with markers for OC + oligodendrocyte precursor cell (OPC) (Olig2), OC <t>(CC1),</t> OPC (PDGFRα, NG2), neuron (NeuN), astrocyte (S100β) (Olig2 [83.41 ± 3.899%], CC1 [72.65 ± 3.464%], PDGFRα+ [9.979 ± 0.9322%], NG2 [6.736 ± 0.2401%], NeuN [1.481 ± 0.7246%], and S100β [8.156 ± 0.5504%]). Scale bar, 30 μm. (C,D) Representative images of MBP immunostaining in the motor cortex of PLP-GCaMP6 mice. Scale bars: in (C) , 100 μm; in (D) , 30 μm. (E) Quantitative analysis of the co-localization areas of MBP and GCaMP’ + ve processes based on immunostaining data. The proportion of MBP’ + ve processes in the processes of GCaMP’ + ve cells was about 15%. (F) Experimental protocol of Ca 2+ imaging in OCs. The first craniotomy was performed one week before Pre-imaging (Pre) using two-photon microscopy. After Pre-imaging, a second craniotomy was performed and TTX was applied for 30 min, followed by a second imaging (TTX) of the same cells. (G,H) Representative image of GCaMP’ + ve cells of the motor cortex in PLP-GCaMP6 mice before and after TTX application. Red spots indicate the Ca 2+ activated areas (spot). Scale bar = 30 μm. Representative Ca 2+ traces from spots of typical GCaMP’ + ve cells are shown. (I) Changes in Ca 2+ spots, Ca 2+ events and total area under the curve (AUC) of GCaMP’ + ve cells between before and after TTX application. The number of Ca 2+ spots and Ca 2+ events and total AUC were significantly decreased after TTX application. Pre: n = 6 mice, 13 imaging fields (cells); TTX: n = 6 mice, 13 imaging fields (cells). * p < 0.05, ** p < 0.01, Mann–Whitney U test. Data are presented as mean ± standard error of mean. For detailed data, check the source data file. (J) Changes in AUC, Amplitude, and Latency of GCaMP’ + ve cells between before and after TTX application. AUC was not significantly changed, Amplitude was significantly decreased, and Latency was significantly increased after TTX application. Pre: n = 6 mice, 426 events; TTX: n = 6 mice, 189 events. N.S., not significant, * p < 0.05, *** p < 0.001, Mann–Whitney U test. Violin plots show median (black line) and distribution of the data. For detailed data, check the source data file. (K) Proportions of AUC and Latency were not significantly changed between before and after TTX application. The proportion of lower Amplitude was significantly increased after TTX application. N.S., not significant, ** p < 0.01, Kolmogorov–Smirnov test. For detailed data, check the source data file.
Aif 1/Iba1 Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Journal: eLife

Article Title: CNS myelination and remyelination depend on fatty acid synthesis by oligodendrocytes

doi: 10.7554/eLife.44702

Figure Lengend Snippet:

Article Snippet: The following antibodies were used: Rabbit anti-cleaved Caspase 3 (IHC 1:500, Cat# 9661, Cell signaling Technology, Danvers, MA, USA; RRID: AB_2341188 ), mouse anti-CC1 (IHC 1:200 in the analysis of development, 1:300 in the analysis of remyelination, Cat# OP80, Merck Millipore, Billerica, MA, USA; RRID: AB_2057371 ), rabbit anti-FASN (IHC 1:200, Cat# Ab22759, Abcam, UK; RRID: AB_732316 ), chicken anti-GFP (IHC 1:1000, Cat# Ab13970, Abcam, UK; RRID: AB_300798 ), mouse anti-ki67 (IHC 1:200, Cat# m7249, clone MIB-5, Dako Agilent, Santa Clara, CA, USA; RRID: AB_2250503 ), rat anti-MBP (IHC 1:200 in the analysis of development, IHC 1:300 in the analysis of remyelination, Cat# MCA409S, Serotec/BioRad Laboratories, Hercules, CA, USA; RRID: AB_325004 ), goat anti-Olig2 (IHC 1:25, Cat# AF2418, R and D Systems, Minneapolis, MN, USA; RRID: AB_2157554 ; biotinylated), mouse anti-Olig2 (IHC 1:1000, clone 211F1.1, Cat# MABN50, Merck Millipore, Billerica, MA, USA; RRID: AB_10807410 ), rabbit anti-Olig2 (IHC 1:500 in the analysis of development, IHC 1:400 in the analysis of remyelination, Cat# AB9610, Merck Millipore, Billerica, MA, USA; RRID: AB_570666 ), rabbit anti-PDGFRα (IHC 1:500, Cat# 3174, Cell signaling Technology, Danvers, MA, USA; RRID: AB_2162345 ), donkey anti-chicken Alexa488 (IHC 1:1000, Cat# 703-545-155, Jackson ImmunoResearch, West Grove, PA, USA; RRID: AB_2340375 ), goat anti-mouse Alexa488 (IHC 1:300, Cat# A11029, Thermo Fisher Scientific, Waltham, MA, USA; RRID: AB_138404 ), donkey anti-mouse Alexa546 (IHC 1:1000, Cat# A10036, Thermo Fisher Scientific, Waltham, MA, USA; RRID: AB_2534012 ), goat anti-mouse Alexa546 (IHC 1:1000, Cat# A11030, Thermo Fisher Scientific, Waltham, MA, USA; RRID: AB_144695 ), goat anti-mouse Alexa647 (IHC 1:1000, Cat# A21237, Thermo Fisher Scientific, Waltham, MA, USA; RRID: AB_1500743 ), donkey anti-rabbit Alexa488 (IHC 1:1000, Cat# A21206, Thermo Fisher Scientific, Waltham, MA, USA; RRID: AB_2535792 ), donkey anti-rabbit Alexa647 (IHC 1:1000, Cat# A31573, Thermo Fisher Scientific, Waltham, MA, USA; RRID: AB_2536183 ), goat anti-rabbit Alexa546 (IHC 1:1000, Cat# A11035, Thermo Fisher Scientific, Waltham, MA, USA; RRID: AB_143051 ), goat anti-rabbit Alexa594 (IHC 1:300, Cat# A11012, Thermo Fisher Scientific, Waltham, MA, USA; RRID: AB_141359 ), goat anti-rabbit Alexa647 (IHC 1:1000, Cat# A21244, Thermo Fisher Scientific, Waltham, MA, USA; RRID: AB_141663 ), goat anti-rat Alexa647 (IHC 1:300, Cat# A21247, Thermo Fisher Scientific, Waltham, MA, USA; RRID: AB_141778 ), Streptavidin-Alexa647 (IHC 1:1000, Cat# S21374, Thermo Fisher Scientific, Waltham, MA, USA; RRID: AB_2336066 ).

Techniques: Sequencing, Transgenic Assay, EdU Assay, Blocking Assay, Plasmid Preparation, Sample Prep, Software

Neuronal activity-dependent Ca 2+ activities in oligodendrocytes (OCs). (A,B) Representative images (A) and quantification (B) of GCaMP expression in the motor cortex of PLP-GCaMP mice and co-localization with markers for OC + oligodendrocyte precursor cell (OPC) (Olig2), OC (CC1), OPC (PDGFRα, NG2), neuron (NeuN), astrocyte (S100β) (Olig2 [83.41 ± 3.899%], CC1 [72.65 ± 3.464%], PDGFRα+ [9.979 ± 0.9322%], NG2 [6.736 ± 0.2401%], NeuN [1.481 ± 0.7246%], and S100β [8.156 ± 0.5504%]). Scale bar, 30 μm. (C,D) Representative images of MBP immunostaining in the motor cortex of PLP-GCaMP6 mice. Scale bars: in (C) , 100 μm; in (D) , 30 μm. (E) Quantitative analysis of the co-localization areas of MBP and GCaMP’ + ve processes based on immunostaining data. The proportion of MBP’ + ve processes in the processes of GCaMP’ + ve cells was about 15%. (F) Experimental protocol of Ca 2+ imaging in OCs. The first craniotomy was performed one week before Pre-imaging (Pre) using two-photon microscopy. After Pre-imaging, a second craniotomy was performed and TTX was applied for 30 min, followed by a second imaging (TTX) of the same cells. (G,H) Representative image of GCaMP’ + ve cells of the motor cortex in PLP-GCaMP6 mice before and after TTX application. Red spots indicate the Ca 2+ activated areas (spot). Scale bar = 30 μm. Representative Ca 2+ traces from spots of typical GCaMP’ + ve cells are shown. (I) Changes in Ca 2+ spots, Ca 2+ events and total area under the curve (AUC) of GCaMP’ + ve cells between before and after TTX application. The number of Ca 2+ spots and Ca 2+ events and total AUC were significantly decreased after TTX application. Pre: n = 6 mice, 13 imaging fields (cells); TTX: n = 6 mice, 13 imaging fields (cells). * p < 0.05, ** p < 0.01, Mann–Whitney U test. Data are presented as mean ± standard error of mean. For detailed data, check the source data file. (J) Changes in AUC, Amplitude, and Latency of GCaMP’ + ve cells between before and after TTX application. AUC was not significantly changed, Amplitude was significantly decreased, and Latency was significantly increased after TTX application. Pre: n = 6 mice, 426 events; TTX: n = 6 mice, 189 events. N.S., not significant, * p < 0.05, *** p < 0.001, Mann–Whitney U test. Violin plots show median (black line) and distribution of the data. For detailed data, check the source data file. (K) Proportions of AUC and Latency were not significantly changed between before and after TTX application. The proportion of lower Amplitude was significantly increased after TTX application. N.S., not significant, ** p < 0.01, Kolmogorov–Smirnov test. For detailed data, check the source data file.

Journal: Frontiers in Cellular Neuroscience

Article Title: Activity-dependent oligodendrocyte calcium dynamics and their changes in Alzheimer’s disease

doi: 10.3389/fncel.2023.1154196

Figure Lengend Snippet: Neuronal activity-dependent Ca 2+ activities in oligodendrocytes (OCs). (A,B) Representative images (A) and quantification (B) of GCaMP expression in the motor cortex of PLP-GCaMP mice and co-localization with markers for OC + oligodendrocyte precursor cell (OPC) (Olig2), OC (CC1), OPC (PDGFRα, NG2), neuron (NeuN), astrocyte (S100β) (Olig2 [83.41 ± 3.899%], CC1 [72.65 ± 3.464%], PDGFRα+ [9.979 ± 0.9322%], NG2 [6.736 ± 0.2401%], NeuN [1.481 ± 0.7246%], and S100β [8.156 ± 0.5504%]). Scale bar, 30 μm. (C,D) Representative images of MBP immunostaining in the motor cortex of PLP-GCaMP6 mice. Scale bars: in (C) , 100 μm; in (D) , 30 μm. (E) Quantitative analysis of the co-localization areas of MBP and GCaMP’ + ve processes based on immunostaining data. The proportion of MBP’ + ve processes in the processes of GCaMP’ + ve cells was about 15%. (F) Experimental protocol of Ca 2+ imaging in OCs. The first craniotomy was performed one week before Pre-imaging (Pre) using two-photon microscopy. After Pre-imaging, a second craniotomy was performed and TTX was applied for 30 min, followed by a second imaging (TTX) of the same cells. (G,H) Representative image of GCaMP’ + ve cells of the motor cortex in PLP-GCaMP6 mice before and after TTX application. Red spots indicate the Ca 2+ activated areas (spot). Scale bar = 30 μm. Representative Ca 2+ traces from spots of typical GCaMP’ + ve cells are shown. (I) Changes in Ca 2+ spots, Ca 2+ events and total area under the curve (AUC) of GCaMP’ + ve cells between before and after TTX application. The number of Ca 2+ spots and Ca 2+ events and total AUC were significantly decreased after TTX application. Pre: n = 6 mice, 13 imaging fields (cells); TTX: n = 6 mice, 13 imaging fields (cells). * p < 0.05, ** p < 0.01, Mann–Whitney U test. Data are presented as mean ± standard error of mean. For detailed data, check the source data file. (J) Changes in AUC, Amplitude, and Latency of GCaMP’ + ve cells between before and after TTX application. AUC was not significantly changed, Amplitude was significantly decreased, and Latency was significantly increased after TTX application. Pre: n = 6 mice, 426 events; TTX: n = 6 mice, 189 events. N.S., not significant, * p < 0.05, *** p < 0.001, Mann–Whitney U test. Violin plots show median (black line) and distribution of the data. For detailed data, check the source data file. (K) Proportions of AUC and Latency were not significantly changed between before and after TTX application. The proportion of lower Amplitude was significantly increased after TTX application. N.S., not significant, ** p < 0.01, Kolmogorov–Smirnov test. For detailed data, check the source data file.

Article Snippet: The primary antibodies used in this study were as follows: anti-Olig2 (rabbit; Millipore, AB9610; 1:1000), anti-adenomatous polyposis coli (clone CC1) (mouse; Calbiochem, OP80; 1:500), anti-PDGFRα (goat; R&D systems, AF1062; 1:200), anti-NG2 (rabbit; Millipore, AB5320; 1:500), anti-NeuN (mouse; Millipore, MAB377; 1:500), anti-S100β (rabbit; Abcam, ab52642; 1:1000), anti-MBP (mouse; BioLegend, clone SMI 99; 1:100), and anti-GFP (chicken; Novus biologicals, NB100-1614; 1:2000).

Techniques: Activity Assay, Expressing, Immunostaining, Imaging, Microscopy, MANN-WHITNEY